Device

Part:BBa_J44005:Design

Designed by: Todd Eckdahl   Group: iGEM06_Missouri   (2006-08-22)


pLac-RBS-Hin-TT-HixC-pBAD-HixC-RBS-TetF-TT-RE


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1106
    Illegal NheI site found at 1318
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1046
    Illegal BamHI site found at 1464
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1490
    Illegal NgoMIV site found at 1858
    Illegal NgoMIV site found at 2018
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Possibility of read-thru of the pBAD during transcription.

Source

(BBa_B0030), (BBa_B0015), (BBa_R0010), (BBa_I13453), (BBa_J31007), (BBa_J3101)- from registry. (BBa_J31000) - from Davidson College. (BBa_J44000) - made in collaboration with Missouri Western/Davidson.

References